GOGAT | Glutamine oxoglutarate aminotransferase

Cat: PA00461

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Miscanthus giganteus, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Populus tremula, Solanum lycopersicum, Spartina sp., Zea mays

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide well conserved in known GOGAT sequences from different species including Arabidopsis thaliana Fd-GOGAT 1 Q9ZNZ7, At5g04140 and Fd-GOGAT 2 Q9T0P4, At2g41220

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

177 | 170-180 kDa depending upon the species

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Miscanthus giganteus, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Populus tremula, Solanum lycopersicum, Spartina sp., Zea mays

Predicted reactivity

Arthrospira platensis PCC 7345, Beta vulgaris, Brachypodium distachyon, Burkholderia glumae, Camellia sinensis, Chloroflexi bacterium, Chlamydonas reinhardtii, Gracilaria tenustipitata, Cyanobacteria, Crocosphaera watsonii WH 8502, Cyanidioschyzon merolae (strain 10D), Galdieria sulphuraria , Glycine max, Helicosporidium sp. ATCC 50920, Hydrogenobaculum sp. HO, Lactococcus lactis subsp. lactis bv. diacetylactis str. TIFN2, Leptospira interrogans serovar Bataviae str. HAI135, Leptolyngbya boryana, Medicago trucutula, Microcystis panniformis, Monoraphidium neglectum, Morus notabilis, Nannochloropsis gaditana, Nicotiana tabacum, Ostreococcus lucimarinus,Physcomitrium patens subsp. patens, Porphyra purpurea, Sutterella wadsworthensis, Sulfurihydrogenibium yellowstonense, Veillonella atypica

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

This antibody can be used as a plastidial marker for leaf material, not roots where levels of GOGAT are too low. A 40 kDa band present in A. thaliana sample is not competed away during antibody neutralization assay. In this assay free peptide used for antibody production is incubated together with anti-GOGAT antibodies. Due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time.

Description

Glutaminoxy-glutarate aminotransferase (GOGAT) is an enzyme involved in the synthesis of glutamate from glutamine and alpha-ketoglutarate. There are two forms of GOGAT in plants: ferridoxin dependent GOGAT (Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT). 95% of GOGAT in plants are Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 and glu2(in Arabidopsis), located on chromosomes 5 and 2. Fd-GOGAT(both forms) is highly conserved in plants, red algae and cyanobacteria.

For research use only, not for clinical use.