Goat anti-biotin, HRP conjugated

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Cat: PA00123

Size: 1 mg

Host: Goat

Clonality: Polyclonal

Datasheet:

Product Info

Immunogen

Biotin

Host

Goat

Clonality

Polyclonal

Purity

Immunogen affinity purified goat IgG.

Format

Lyophilized

Reconstitution

For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage

Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Recommended dilution

This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,

Reactivity

Additional information

Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,0 mg/ml, Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2,1 % (w/v) B, Protease/IgG free, Contains 0,1 % (v/v) Kathon CG as preservative of bacterial growth

Description

Goat antibiotin is a secondary antibody that binds to HRP and binds to biotin in immunoassay. Antibodies are purified by solid phase biotin affinity.

For research use only, not for clinical use.