GN | Gnom

Inquiry

Cat: PA00701

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana GN protein sequence, UniProt: Q42510, TAIR: At1g13980

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

162 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Nitrocellulose membrane with pore size 0.45 µm is recommended as well as TBS-T as antibody incubation buffer. This antibody is also recognzing recombinant GN fused to GFP.

Description

GN (gnm) activates ARF proteins by exchanging bound GDP for free GTP and plays a role in vesicular protein sorting. It acts as a major regulator of endosomal vesicle transport, but is also involved in endocytosis. GN is necessary for proper cell wall organization and normal cell adhesion during seedling development, and also plays an important role in the hydrophilicity of seedling roots. Other names: Pattern forming protein EMB30, protein embryo defect 30, protein MIZU-KUSSEI2, protein vascular network 7

For research use only, not for clinical use.