GltBD | NADPH-dependent glutamate synthase

Inquiry

Cat: PA00630

Size: 200 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Synechocystis sp. strain PCC6803

Datasheet:

Product Info

Immunogen

Purified full length, tag cleaved, recombinant cyanobacterium, Leptolyngbya boryana (Plectonema boryanum) , glutamate synthase, UniProt: Q51583 (gltB, large subunit L.boryanum), Q51584 (gltD, small subunit L.boryanum)

Host

Rabbit

Clonality

Polyclonal

Purity

Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.

Format

Liquid at 4 mg/ml.

Storage

Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

ELISA (ELISA), Western blot (WB)

Recommended dilution

1: 1000 - 1: 2000 (WB)

Expected | apparent MW

167.9 | 168 kDa (large subunit)54.2 | 54 kDa (small subunit)

Reactivity

Confirmed reactivity

Synechocystis sp. strain PCC6803

Predicted reactivity

cyanobacteria

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

This antibody is recognizing both, large (GltB) and small (GltD) subunits of NADPH-dependent glutamate synthase

Description

Nadh-dependent glutamate synthetase (GltBD) is involved in the biosynthesis of glutamate and consists of a large subunit (GltB, 168 kDa) and a small subunit (GltD, 54 kDa). It is necessary for non-photorespiratory ammonia assimilation.

For research use only, not for clinical use.