GlnA | Glutamine synthetase

Cat: PA01106

Size: 50 µl (16 mg/ml)

Host: Chicken

Clonality: Polyclonal

Confirmed reactivity: Deinococcus radioduransm Synechococcus sp. strain PCC 7942, Synechocystis sp. strain PCC 6803, Trichodesmium IMS

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide derived from available bacterial GlnA sequences with perfect conservation in alpha, beta, gamma Proteobacteria, Enterobacteria, Thermotogales, Low GC Gram+, Cyanobacteria (except weak conservation with Trichodesmium thiebautii) including Synechocystis PCC 6803 Q59981

Host

Chicken

Clonality

Polyclonal

Purity

Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Format

Liquid

Storage

Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 5000 (WB)

Expected | apparent MW

53 kDa

Reactivity

Confirmed reactivity

Deinococcus radioduransm Synechococcus sp. strain PCC 7942, Synechocystis sp. strain PCC 6803, Trichodesmium IMS

Predicted reactivity

Alpha, beta, gamma proteobacteria, Arthropsira sp. PCC 8005, Crenarchaeotes, Enterobacteria, Escherichia coli, Euryarchaeotes, Thermotogales

Not reactive in

Diatoms, eukaryotic GlnA

Additional information

Peptide target used to elicit this antibody has a weak, sporadic conservation with Glutamine Synthetase to III, antibody not expected to detect this enzyme, Weak conservation with some Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GLNRS), but this antibody is not expected to detect this enzyme

Description

Glutamine synthetase (EC=6.3.1.2) is a key enzyme in the synthesis of mineral nitrogen by glutamine. When glutamine is abundant, the activity of this enzyme is controlled by adenylate.

For research use only, not for clinical use.