GLN2 | GS2, chloroplastic form of glutamine synthetase

Inquiry

Cat: PA01223

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide which is a part of part of the glutamine synthetase/guanido kinase superfamily catalytic region chosen from various available sequences, including Arabidopsis thaliana GLN2, UniProt: Q43127, TAIR: AT5G35630

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

ImmunoGold (IG), (Western blot (WB)

Recommended dilution

1 : 5000 on 0,5-5 ug protein/lane detection (WB)

Expected | apparent MW

47 | 44-45 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea

Predicted reactivity

Brassica napus, Diplotaxis tenuifolia,Eruca versicaria, Glycine max, Hordeum vulgare, Medicago truncatula, Pinus sylvestris, Phaseolus vulgaris, Physcomitrium patens, Populus sp., Triticum aestivum, Zea mays

Not reactive in

Diatoms

Additional information

This product can be sold contacining proclin if requested

Description

Glutamine synthetase (GLN or GS) is one of the key enzymes involved in plant nitrogen metabolism. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two main classes of glutamine synthetases in plants :GLN1 is the cytoplasmic form and GLN2 is the chloroplast form. GLN2 is encoded by a single gene and is abundant in leaf mesophyll cells for the absorption of ammonia produced by photorespiration and the reduction of nitrates in chloroplasts. GLN2 is the target of thioredoxin.

For research use only, not for clinical use.