GFP | Green Fluorescence Protein (total IgY)

Inquiry

Cat: PA00046

Size: 100 µl (1 mg/ml)

Host: Chicken

Clonality: Polyclonal

Confirmed reactivity: Native GFP, Recombinant GFP (E,coli), all variants of GFP

Datasheet:

Product Info

Immunogen

highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212

Host

Chicken

Clonality

Polyclonal

Purity

Total IgY in 10 mM TRIS, 0,15 mM NaCl, pH 8. Contains 0.02 % sodium azide.

Format

Liquid

Storage

Store at 4°C; make aliquots to avoid working with a stock. Or Store in small aliquots at -20°C, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

ELISA (ELISA), Western blot (WB)

Recommended dilution

1 : 5000-1 : 25 000 (ELISA), 1 : 2000-1 : 10 000 (WB)

Reactivity

Confirmed reactivity

Native GFP, Recombinant GFP (E,coli), all variants of GFP

Additional information

Minimal cross-reactivity with E.coli proteins

Description

Green fluorescent protein (GFP) is originally found in the light organs of the jellyfish (Aequorea victoria). It is a naturally occurring fluorescent protein that, when excited by blue or ultraviolet light, emits green light with a maximum wavelength of 509 nanometers. It is widely used in the laboratory as a reporter molecule for labeling and studying cellular and subcellular proteins in living cells, with a wide range of uses. GFP protein antibodies are used in western blotting and ELISA. The molecular weight of GFP protein is 27 kDa.

For research use only, not for clinical use.