GFP | Green Fluorescence Protein (protein A purified)
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GFP | Green Fluorescence Protein (protein A purified)

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Cat: PA00045
Size: 100 µg at 1 mg/ml
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Native GFP, Recombinant GFP (E.coli), all variants of GFP and EGFP
Datasheet:

Product Info

Immunogen
highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG. Protein A purified.
Format
Liquid
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
ELISA (ELISA), Western blot (WB)
Recommended dilution
1 : 5000-1 : 25 000 (ELISA), 1 : 2000-1 : 10 000 (WB)

Reactivity

Confirmed reactivity
Native GFP, Recombinant GFP (E.coli), all variants of GFP and EGFP

Additional information

Minimal cross-reactivity with E.coli proteins

Description

Green fluorescent protein (GFP) is originally found in the light organs of the jellyfish (Aequorea victoria). It is a naturally occurring fluorescent protein that, when excited by blue or ultraviolet light, emits green light with a maximum wavelength of 509 nanometers. It is widely used in the laboratory as a reporter molecule for labeling and studying cellular and subcellular proteins in living cells, with a wide range of uses. GFP protein antibodies are used in western blotting and ELISA. The molecular weight of GFP protein is 27 kDa.

For research use only, not for clinical use.