GFP | Green Fluorescence Protein, monoclonal (clone 2G4:F2)

Inquiry

Cat: PA00055

Size: 50 µg

Host: Mouse

Clonality: Monoclonal

Confirmed reactivity: GFP-tagged proteins

Datasheet:

Product Info

Immunogen

Recombinant GFP protein derived from Aequorea victoria, UniProt: P42212

Host

Mouse

Clonality

Monoclonal

Purity

Purified mouse IgG in PBS pH 7.4

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes

Application

Western blot (WB)

Recommended dilution

1: 1000 - 1 : 30 000 (WB)

Expected | apparent MW

Depends upon fusion partner

Reactivity

Confirmed reactivity

GFP-tagged proteins

Description

Green fluorescent protein (GFP) is originally found in the light organs of the jellyfish (Aequorea victoria). It is a naturally occurring fluorescent protein that, when excited by blue or ultraviolet light, emits green light with a maximum wavelength of 509 nanometers. It is widely used in the laboratory as a reporter molecule for labeling and studying cellular and subcellular proteins in living cells, with a wide range of uses. GFP protein antibodies are used in western blotting and ELISA. The molecular weight of GFP protein is 27 kDa.

For research use only, not for clinical use.