GFP | Green Fluorescence Protein, clone 1A5 (rat monoclonal)

Inquiry

Cat: PA00054

Size: 100 µg

Host: Rat

Clonality: Monoclonal

Confirmed reactivity: Native GFP, Recombinant GFP (E,coli), all variants of GFP, including EGFP

Datasheet:

Product Info

Immunogen

Recombinant GFP protein from Aequorea victoria, UniProt: P42212

Host

Rat

Clonality

Monoclonal

Purity

Immunoglobulin Protein A purified in PBS, 50 % glycerol, filter sterilized.

Format

Liquid, 1mg/ml.

Storage

Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Chromatin immunoprecipitation (ChIP), ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution

1 : 5000-1 : 25 000 (ELISA), 1 : 500 (IF), 1 : 2000-1 : 10 000 (WB)

Reactivity

Confirmed reactivity

Native GFP, Recombinant GFP (E,coli), all variants of GFP, including EGFP

Additional information

Antibody detects GFP and EGFP

Description

Green fluorescent protein (GFP) is originally found in the light organs of the jellyfish (Aequorea victoria). It is a naturally occurring fluorescent protein that, when excited by blue or ultraviolet light, emits green light with a maximum wavelength of 509 nanometers. It is widely used in the laboratory as a reporter molecule for labeling and studying cellular and subcellular proteins in living cells, with a wide range of uses. GFP protein antibodies are used in western blotting and ELISA. The molecular weight of GFP protein is 27 kDa.

For research use only, not for clinical use.