GFP | Green Fluorescence Protein (affinity purified)

Inquiry

Cat: PA00044

Size: 100 µg at 1 mg/ml

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Native GFP, Recombinant GFP (E,coli), all variants of GFP, including EGFP

Datasheet:

Product Info

Immunogen

Highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Liquid

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution

1 : 5000-1 : 25 000 (ELISA), 1: 100 - 1 : 500 (IF), 1 : 2000-1 : 10 000 (WB)

Reactivity

Confirmed reactivity

Native GFP, Recombinant GFP (E,coli), all variants of GFP, including EGFP

Additional information

10 mM TRIS buffer pH 8,0 containing 0,02% sodium azide,Minimal cross-reactivity with E.coli proteins. Antibody reactivity to mVenus or EBFP2 has not yet been determined.

Description

Green fluorescent protein (GFP) is originally found in the light organs of the jellyfish (Aequorea victoria). It is a naturally occurring fluorescent protein that, when excited by blue or ultraviolet light, emits green light with a maximum wavelength of 509 nanometers. It is widely used in the laboratory as a reporter molecule for labeling and studying cellular and subcellular proteins in living cells, with a wide range of uses. GFP protein antibodies are used in western blotting and ELISA. The molecular weight of GFP protein is 27 kDa.

For research use only, not for clinical use.