GDP-L-Galactose Phosphorylase

Cat: PA00539

Size: 200 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Brassica oleracea var. italica, Brassica rapa var. komatsuna, Citrus limon, Nicotiana tabacum, Spinacia oleracea, Zea mays, Chlamydomonas reinhardtii

Datasheet:

Product Info

Immunogen

KLH-conjuated synthetic peptide derived from known GDP-L-Galactose Phosphorylase sequences, including Arabidopsis thaliana Q8LKQ7 (At4g26850) and Chlamydomonas reinhardtii

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 200 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1200 , 1 : 3000 (WB)

Expected | apparent MW

51 | 50 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Brassica oleracea var. italica, Brassica rapa var. komatsuna, Citrus limon, Nicotiana tabacum, Spinacia oleracea, Zea mays, Chlamydomonas reinhardtii

Predicted reactivity

Manihot esculenta, Sorghum bicolor, Oryza sativa, Physcomitrium patens

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Description

Gdp-l-galactose phosphorylase is a histidine trimer (HIT) enzyme involved in the Smirnoff Wheeler pathway of plant ascorbic acid synthesis. It is encoded by the VTC2 gene. This enzyme catalyzes the conversion of GDP-L-galactose to L-galactose 1-phosphate, consuming inorganic phosphate and producing GDP.

For research use only, not for clinical use.