Products
Online Inquiry
GDP-L-galactose phosphorylase 1 (VTC2), Recombinant Protein
Cat: RP02157
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Yeast)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana GDP-L-galactose phosphorylase 1 (VTC2)
Product Gene Name
VTC2 recombinant protein
Product Synonym Gene Name
VTC2
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MLKIKRVPTV VSNYQKDDGA EDPVGCGRNC LGACCLNGAR LPLYACKNLV KSGEKLVISH EAIEPPVAFL ESLVLGEWED RFQRGLFRYD VTACETKVIP GKYGFVAQLN EGRHLKKRPT EFRVDKVLQS FDGSKFNFTK VGQEELLFQF EAGEDAQVQF FPCMPIDPEN SPSVVAINVS PIEYGHVLLI PRVLDCLPQR IDHKSLLLAV HMAAEAANPY FRLGYNSLGA FATINHLHFQ AYYLAMPFPL EKAPTKKITT TVSGVKISEL LSYPVRSLLF EGGSSMQELS DTVSDCCVCL QNNNIPFNIL ISDCGRQIFL MPQCYAEKQA LGEVSPEVLE TQVNPAVWEI SGHMVLKRKE DYEGASEDNA WRLLAEASLS EERFKEVTAL AFEAIGCSNQ EEDLEGTIVH QQNSSGNVNQ KSNRTHGGPI TNGTAAECLV LQ
Sequence Positions
1-442, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
48,970 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
GDP-L-galactose phosphorylase
NCBI/Uniprot Data
NCBI Accession #
NP_567759.1
NCBI GI #
18416877
NCBI GenBank Nucleotide #
NM_118819.3
NCBI GeneID
828792
NCBI Official Full Name
GDP-L-galactose phosphorylase 1
NCBI Official Symbol
VTC2
NCBI Official Synonym Symbols
F10M23.190; F10M23_190; vitamin c defective 2
NCBI Protein Information
GDP-L-galactose phosphorylase 1
NCBI Summary
Encodes a novel protein involved in ascorbate biosynthesis, which was shown to catalyze the transfer of GMP from GDP-galactose to a variety of hexose-1-phosphate acceptors. Recessive mutation has a reduced amount of vitamin C, lower level of non-photochemical quenching, and reduced rate of conversion of violaxanthin to zeaxanthin in high light.
UniProt Gene Name
VTC2
UniProt Protein Name
GDP-L-galactose phosphorylase 1
UniProt Synonym Protein Names
Protein VITAMIN C DEFECTIVE 2
UniProt Primary Accession #
Q8RWE8
UniProt Secondary Accession #
Q8LKQ7; Q940B4; Q9SZ25
UniProt Related Accession #
Q8RWE8
UniProt Comments
Catalyzes a reaction of the Smirnoff-Wheeler pathway, the major route to ascorbate biosynthesis in plants. Acts as a phosphorylase rather than as a transferase. Uses preferentially GDP-L-galactose and GDP-D-glucose as substrates. Lower activity with GDP-L-fucose, very low activity with GDP-D-mannose, and no activity with UDP-D-glucose, UDP-D-galactose or ADP-D-glucose. Highly specific for inorganic phosphate as the guanylyl acceptor.
For research use only, not for clinical use.