Gamma-glutamyl-cysteine
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Gamma-glutamyl-cysteine

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Cat: PA00794
Size: 2x100 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Cucurbita pepo L. subsp. peop var. styriaca Greb, Nicotiana tabacum cv. samsun
Datasheet:

Product Info

Immunogen
γ-glutamyl-cysteine (gamma-EC) linked by glutaraldehyde
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water per tube
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunocytochemistry (ICC), Immunogold (IG)
Recommended dilution
1 : 50 (ICC), (IG)

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Cucurbita pepo L. subsp. peop var. styriaca Greb, Nicotiana tabacum cv. samsun
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Immunogold labeling for electron microscopy Block ultrathin sections (80nm) prepared for immunogold labeling with 2% bovine serum albumine (BSA) in phosphate buffered saline (PBS, pH 7.2). Then treat the sections with the primary antibody (anti-γ-glutamylcysteine rabbit polyclonal IgG) diluted 1:50 in PBS containing 1% BSA for 2 h at room temperature. After a short rinse in PBS (3 X 5 min), incubate samples with a gold-conjugated secondary antibody (goat anti-rabbit IgG; eg. 10nm) diluted 1:50 in PBS including 1% BSA for 90 min at room temperature. After a short wash in PBS (2 X 5 min), and distilled water (3 X 5 min) observe grids under a transmission electron microscope.

Description

Gamma-glutamylcysteine is a precursor of glutathione and is synthesized by gamma-glutamylcysteine synthase from a chain of glutamate and cysteine. Synonyms :gamma-glutamyl-cysteine, gamma-glutamyl-cysteine. Alias: gamma-EC, gamma-Glu-CYS.

For research use only, not for clinical use.