Gamma-ECS | Gamma glutamylcysteine synthase

Inquiry

Cat: PA00793

Size: 100 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Nicotiana tabcum, Salicornia sp., Solanum lycopersicum, Zea mays

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide derived from C-terminal part of Zea mays gamma-ECS Q8W4W3

Host

Rabbit

Clonality

Polyclonal

Purity

Total IgG. Protein G purified in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunolocalization (IL), Western blot (WB)

Recommended dilution

1 : 5000 (IL), 1 : 5000 (WB)

Expected | apparent MW

50 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Nicotiana tabcum, Salicornia sp., Solanum lycopersicum, Zea mays

Predicted reactivity

Marchantia polymorpha, Pisum sativum, Riciuns communis, Oryza sativa, Triticum aestivum

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Total IgG concentration is 5,2 µg/ µl,Antibodies has been used in immunolocalization on Arabidopsis thaliana.

Description

Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyzes the first step in glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamic acid and cysteine. In the second step, glycine is added to the C-terminal of (gamma-EC) under the catalysis of GSH-S. Glutathione is the main non-protein mercaptan, which acts as an antioxidant in the stress response and acts as a mobile pool to reduce sulfur. It is also important in regulating plant growth and development.

For research use only, not for clinical use.