Galanthus Nivalis Lectin (GNL), Agarose bound

Inquiry

Cat: PL00699

Size: 5 ml

Sugar Specificity: Mannose

Datasheet:

Description

Agarose binding kale flower lectin is prepared from affinity purified lectin. Unlike most mannose-specific lectins, Galanthus nivalis is not a metal protein and does not require ca2 + or N ++ binding. Binding appears to be directed preferentially towards structures containing (alpha-1,3) mannose residues. Contrary to most mannose-binding lectins, GNL does not bind alpha-linked glucose. Reports suggest that this lectin binds IgM in rats and mice, but not IgG. The only protein reported to bind to this lectin in human serum is α2-macroglobulin. GNL binds to many viral glycoproteins.

Product Info

Conjugate

Agarose

Sugar Specificity

Mannose

Storage

2-8℃; Do not freeze

Application

Glycobiology, Affinity Chromatography

Recommended dilution

Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-1100. Alternatively, 0.1 M α methyl mannoside can be used.For those glycoconjugates having a very high affinity for GNL, it may be necessary to lower the pH of the eluting sugar solution to pH 4.0 with acetic acid and increase the concentration of the α methyl mannoside to 0.5 M. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.

Solution

10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.01 mM MnCl2, 20 mM mannose, 0.08% sodium azide

For research use only, not for clinical use.