GAI | DELLA protein GAI

Inquiry

Cat: PA01547

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conugated synthetic peptide, chosen from Arabidopsis thaliana GAI protein sequence UniProt: Q9LQT8, TAIR: At1g14920

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 25 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000 (WB) on transfected Arabidopsis thaliana protoplasts

Expected | apparent MW

58,9 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Not reactive in

Brassica napus, Populus sp., Solanum lycopersicum

Additional information

Homogenization with thiourea and bead beater. Protocol for protein extraction from seeds can be requested here. GAI protein is present in very low levels therefore specific material should be used for analysis as well as chemiluminescence detection reagent in extreme low femtogram range, as AgriseraECLSuperBright.

Description

GAI is a possible transcriptional regulator acting as a suppressor of gibberellin (GA) signaling pathway. Not very sensitive to GA. Synonyms :GRAS family protein 3,AtGRAS-3, gibberellin acid insensitive mutant protein, Growth recovery protein on ammonia 2.

For research use only, not for clinical use.