Total vector size (bp)
13191
Modifications to backbone
Added G10 promoter instead of the 2X35S promoter and added a Glycine Max Codon Optimized Cas9 with an N and C terminus nuclear localization signal.
Bacterial Resistance(s)
Kanamycin, 50 μg/mL
Growth instructions
It may take longer to grow than other plasmids due to the Cas9