Fucose

Alpha (1,3) fucose is present not only in plants but also in some invertebrates (such as nematodes, bees, etc,) , However, cross-reaction with glycoproteins from these organisms is weaker than the one observed in plants, This sugar residue does not exist in mammals, in their endogenous glycoproteins,Controls: PLA2 (phospholipase 2 from bee venom) which contains only α1.3 fucose, Sigma, product number P9279. Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250. The antibody does not recognize alpha 1,6-fucose.

The antibody specifically cross-reacts with a focused residue in α1,3 that binds to the protein N-glycan. This residue is characteristic of plant protein N-glycans and is not present in animal protein N-glycans. This residue is added to the Golgi apparatus.