FtsZ | Procaryotic cell division GTPase (cyanobacterial)

Cat: PA00627

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Cylindrospermopsis raciborskii CS-505, Listeria monocytogenes (weak reaction), Synechococcus elongatus

Datasheet:

Product Info

Immunogen

Whole cyanobacterial (Anabaena PCC 7120) FtsZ protein, UniProt: Q3MC27 overexpressed in E.coli.

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunofluorescence (IF), Immunolocalization (IL), Western blot (WB)

Recommended dilution

1 : 200 (IL) Immunogold-TEM, 1 : 500 (IF), 1 : 2000-1 : 5000 (WB)

Expected | apparent MW

44,5 | 50 kDa

Reactivity

Confirmed reactivity

Cylindrospermopsis raciborskii CS-505, Listeria monocytogenes (weak reaction), Synechococcus elongatus

Predicted reactivity

Phaeodactylum tricornutum, Prochlorococcus sp.

Not reactive in

higher plants

Additional information

To detect E.coli FtsZ protein we recommend a following product: AS10 715 | anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibodyTo detect FtsZ protein in higher plants following antibodies are recommended: AS09 413 | Anti-FtsZ1 and 2 | Plant cell division protein FtsZ1 and FtsZ2, rabbit antibodiesAS13 2651 | Anti-FtsZ2 | Plant cell division protein ftsZ2, rabbit antibodies,This antibody can be used as a loading control antibody in cyanobacteria. Immunofluorescence has been done by labelling Synechococcus elongatus cells at 30°C for 2 hours with FtsZ antibodies diluted to 1: 500 in blocking buffer. Detection images can be found in Kabeya et al (2010).This product can be sold containing ProClin if requested.

Description

FtsZ(Cell Division GTPase) is a characteristic protein of bacterial cell division apparatus. This protein accumulates early in cell division and plays a crucial role in septum formation in most bacteria and chloroplasts. It is also considered to be the bacterial cytoskeletal counterpart of eukaryotic microtubules.

For research use only, not for clinical use.