FtsH1-11 | ATP-dependent zinc metalloprotease FtsH1-11
  1. Home
  2. Products
  3. Plant Antibodies
  4. Plant Structure Related Antibodies
  5. Mitochondria | Respiration
Products
Online Inquiry

FtsH1-11 | ATP-dependent zinc metalloprotease FtsH1-11

Inquiry
Cat: PA01089
Size: 50 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Acetabularia acetabulum, Arabidopsis thaliana, Bryopsis hypnoides (ulvophytes), Guillardia theta, Hordeum vulgare, Synechocystis sp., Thalassiosira pseudonana, Thalassiosira punctigera, Zea mays
Datasheet:

Product Info

Immunogen
KLH-conjugated peptide dereived from sequences of all known FtsH isoforms of Arabidopsis thaliana: FtsH1 Q39102 (At1g50250), FtsH2 O80860 (At2g30950), FtsH3 Q84WU8 (At2g29080), FtsH4 O80983 (At2g26140), FtsH5 Q9FH02 (At5g42270), FtsH6 Q1PDW5 (At5g15250), FtsH7 Q9SD67 (At3g47060), FtsH8 Q8W585 (At1g06430), FtsH9 Q9FIM2 (At5g58870),  FtsH10 Q8VZI8 (At1g07510), FtsH11 Q9FGM0 (At5g53170) as well as 4 FtsH isoforms of Synechocystis sp. PCC6803NP_440330.1, NP_442160.1, NP_440797.1, NP_440525.1
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Blue Native PAGE (BN-PAGE), Western blot (WB)
Recommended dilution
1 : 500-1 : 2000 (WB)
Expected | apparent MW
A. thaliana: FtsH1:76.7, FtsH2: 74, FtsH5: 75.2, FtsH6: 74.5, FtsH7: 87.8, FtsH8: 73, FtsH9:87.8 kDa (chloroplastic); FtsH3: 89.3, FtsH4: 77.2, FtsH10: 89.5 kDa, FtsH11:88.7 kDa (mitochondrial)

Reactivity

Confirmed reactivity
Acetabularia acetabulum, Arabidopsis thaliana, Bryopsis hypnoides (ulvophytes), Guillardia theta, Hordeum vulgare, Synechocystis sp., Thalassiosira pseudonana, Thalassiosira punctigera, Zea mays
Predicted reactivity
Diatoms including Phaeodactylum tricornutum, dicots, Chlamydomonas reinhardtii, Monocots; Nannochloropsis gaditana, Solanum lycopersicum
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

For detection on a diatom samples, load of 5-10 µg/well is required as well as usage of enchanced chemiluminescence. The antibody also detects the chloroplast-encoded FtsH from Thalassiosira pseudonana (diatom) along with 3 related isoforms encode in the nuclear genome of Thalassiosira pseudonana.For detection in BlueNative, recommened dilution is 1: 500.

Description

FtsH belongs to the ATP-dependent peptidase family. In chloroplast positioning has the following subtypes: FTSH1 (synonyms for AAA, FTSH FTSH protease 1), Ftsh2 (VAR2, vargated 2), FtsH5 (VAR1, vargated 1), FtsH6 FTSH protease (6), FtsH7, FtsH8. FtsH9. The following subtypes are localized in mitochondria :FtsH3 (FTSHprotease 3), FtsH4, FtsH10, FtsH11.

For research use only, not for clinical use.