Flap endonuclease 1 (FEN1), Recombinant Protein

Cat: RP01575

Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)

Species: Arabidopsis thaliana (Mouse-ear cress)

Datasheet:

Product Info

Full Product Name

Recombinant Arabidopsis thaliana Flap endonuclease 1 (FEN1)

Product Gene Name

FEN1 recombinant protein

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

MGIKGLTKLL ADNAPSCMKE QKFESYFGRK IAVDASMSIY QFLIVVGRTG TEMLTNEAGE VTSHLQGMFN RTIRLLEAGI KPVYVFDGKP PELKRQELAK RYSKRADATA DLTGAIEAGN KEDIEKYSKR TVKVTKQHND DCKRLLRLMG VPVVEATSEA EAQCAALCKS GKVYGVASED MDSLTFGAPK FLRHLMDPSS RKIPVMEFEV AKILEELQLT MDQFIDLCIL SGCDYCDSIR GIGGQTALKL IRQHGSIETI LENLNKERYQ IPEEWPYNEA RKLFKEPDVI TDEEQLDIKW TSPDEEGIVQ FLVNENGFNI DRVTKAIEKI KTAKNKSSQG RLESFFKPVA NSSVPAKRKE IPESTTKGAA NKKTKGAGGR KKK

Sequence Positions

1-383, Full length protein

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

42,797 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

NCBI/Uniprot Data

NCBI Accession #

NP_001154740.1

NCBI GI #

238481386

NCBI GenBank Nucleotide #

NM_001161268.2

NCBI GeneID

832721

NCBI Official Full Name

5'-3' exonuclease family protein

NCBI Official Symbol

AT5G26680

NCBI Protein Information

5'-3' exonuclease family protein

UniProt Gene Name

FEN1

UniProt Synonym Gene Names

FEN-1

UniProt Protein Name

Flap endonuclease 1

UniProt Synonym Protein Names

Flap structure-specific endonuclease 1

UniProt Primary Accession #

O65251

UniProt Related Accession #

O65251

UniProt Comments

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

For research use only, not for clinical use.