Flap endonuclease 1-A (FEN1a), Recombinant Protein

Full Product Name

Recombinant Oryza sativa subsp. indica Flap endonuclease 1-A (FEN1a)

Product Gene Name

FEN1a recombinant protein

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

MGIKGLTKLL ADNAPKAMKE QKFESYFGRR IAVDASMSIY QFLIVVGRTG METLTNEAGE VTSHLQGMFN RTIRLLEAGI KPVYVFDGKP PDLKKQELAK RYSKREDATK ELTEAVEEGD KDAIEKFSKR TVKVTKQHNE ECKRLLRLMG VPVVEAPCEA EAECAALCIN DMVYAVASED MDSLTFGAPR FLRHLMDPSS KKIPVMEFEV AKVLEELELT MDQFIDLCIL SGCDYCDSIK GIGGQTALKL IRQHGSIESI LENINKDRYQ IPEDWPYQEA RRLFKEPNVT LDIPELKWNA PDEEGLVQFL VKENGFNQDR VTKAIEKIKF AKNKSSQGRL ESFFKPVVST SVPLKRKDTS EKPTKAVANK KTKGAGGKKK

Sequence Positions

1-380, Full length protein

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

42,791 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

NCBI Accession #

B8AW67.1

NCBI GI #

317374863

NCBI Official Full Name

Flap endonuclease 1-A

UniProt Gene Name

FEN1a

UniProt Synonym Gene Names

FEN-1-A

UniProt Protein Name

Flap endonuclease 1-A

UniProt Synonym Protein Names

Flap structure-specific endonuclease 1-A

UniProt Primary Accession #

B8AW67

UniProt Comments

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.