FITC | fluorescein isothiocyanate, Biotin-conjugated (monoclonal, Clone NI 239)

Inquiry

Cat: PA00185

Size: 200 µg

Host: Mouse

Clonality: Monoclonal

Confirmed reactivity: FITC molecule

Datasheet:

Product Info

Immunogen

Highly purified fluorescein isothiocyanate isomer 1,

Host

Mouse

Clonality

Monoclonal

Purity

Purified IgG.1 in PBS pH 7.2, biotin conjugated.

Format

Lyophilized

Reconstitution

For reconstitution add 0.5 ml of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.

Application

ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC) paraffin, Dot blot (Dot), Western blot (WB)

Recommended dilution

The optimum working dilution should be determined by titration by end user. 1:500 (ELISA), 1:50 to 1:200 (ICC, IHC), 1: 1000 (WB).

Reactivity

Confirmed reactivity

FITC molecule

Additional information

This product is intended FOR RESEARCH USE ONLY,This product has not been tested for cross-reactivity with other fluorescent compounds

Description

FITC is a fluorescent dye that absorbs ultraviolet or blue light, causing the molecules to be excited and emit a visible yellow-green light. This emission stops when the light causing the excitation is removed. FITC antibodies are used to enhance the specific signaling of FITC-coupled monoclonal antibodies or secondary antibodies. The sensitivity of the FITC hapten-antihapten system makes it a useful alternative to other systems.

For research use only, not for clinical use.