FeSOD | Chloroplastic Fe-dependent superoxide dismutase

Cat: PA00551

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Armeria maritima, Arabidopsis thaliana, Brassica juncea, Chlamydomonas reinhardtii, Dunaliella bardawil, Dunaliella salina, Fremyella diplosiphon, Medicago sativa, Morus spp., Oryza sativa, Salicornia sp., Solanum tuberosum, winter triticale, Zea mays

Datasheet:

Product Info

Immunogen

overexpressed Chlamydomonas reinhardtii thioredoxine fusion protein A8IGH1, FeSOD excised from a gel piece

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1500-1 : 5000 (WB)

Expected | apparent MW

25 | 22 kDa

Reactivity

Confirmed reactivity

Armeria maritima, Arabidopsis thaliana, Brassica juncea, Chlamydomonas reinhardtii, Dunaliella bardawil, Dunaliella salina, Fremyella diplosiphon, Medicago sativa, Morus spp., Oryza sativa, Salicornia sp., Solanum tuberosum, winter triticale, Zea mays

Predicted reactivity

Algae, Dunaliella salina, Glycine max, Helianthus annuus, Marchantia polymorpha, Nannochloropsis gaditana, Solanum lycopersicum, Physcomitrium patens, Pinus pinaster, Populus balsamifera, Vitis vinifera, Volvox carteri

Additional information

The antibody will detect FeSOD enzyme only in plants grown on low Cu (0.1 μM).Reference: Salah et al (2005) Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. Plant Cell, 17, 1233-1251Out of three FeSOD isoforms, FeSOD2 and FeSOD3 are not expressed in the roots. In roots of Arabidopsis thaliana, FeSOD1 is detected Takáč et al. (2018)This product can be sold containing ProClin if requested

Description

The antioxidant system acts as a defense against oxidative stress. Superoxide dismutase (SOD) catalyzes the conversion of superoxide dismutase into oxygen and hydrogen oxygen. sod is classified as FeSOD, MnSOD or Cu/ZnSOD according to its metal cofactors. Chloroplasts usually contain Cu/ZnSOD and in some plant species contain FeSOD

For research use only, not for clinical use.