Ferritin 1-2 (plant)

Cat: PA00716

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Brassica oleracea, Hordeum vulgare, Medicago truncatula, Pisum sativum, Spinacia oleracea

Datasheet:

Product Info

Immunogen

Purified ferritin from dried peas, Pisum sativum L. After extraction from pea flour, the ferritin is further purified by gel filtration chromatography to >95% purity as estimated from a Coomassie-stained gel.Antibody is most likely to bind to all ferritin isoforms from pea however it has not been confirmed as yet.

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 5000-10 000 (WB)

Expected | apparent MW

23 kDa in legumes, 24 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Brassica oleracea, Hordeum vulgare, Medicago truncatula, Pisum sativum, Spinacia oleracea

Additional information

Note, the calculated molecular weight of pea ferritin is 28 kDa. Removal of the N-terminal targeting sequence upon protein import into plastids results in a protein with an apparent mol weight of ~23 kDa.This antibody is also recognizing horse ferritin (above 100 ng in Western blot).

Description

Ferritin forms a 24-subunit cage for storing the mineral iron. In plants, ferritin is mainly located in plastid, and its expression is upregulated under iron or oxidation stress.

For research use only, not for clinical use.