Fer2 | Ferrritin 2

Inquiry

Cat: PA01180

Size: 200 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Chlamydomonas reinhardtii

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide derived from Fer2 protein sequence (A8WCT5) from Chlamydomonas reinhardtii

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 200 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

32 (with transitpeptide) | 16 (monomer) and ~60 (trimer) for Chlamydomonas reinhardtii

Reactivity

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Chlamydomonas reinhardtii

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

A picture with a western blot application example can be found in the reference given below

Description

Ferritin is the main ferritin storage complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides. In the Chlamydomonas rheinae 2 subtype, ferritin 1 (Fer1) and ferritin 2 (Fer2) are present. Both are plastid localized, but iron-1 is quantitatively recovered in the soluble extract of the cell, while iron-2 is found in the granular part. The partially purified Fer1 complex shows that the two ferriproteins are associated in distinct complexes and do not aggregate. In iron-enriched cells, the ratio of Fer1 to Fer2 is 70:1, suggesting that Fer1 plays a more important role in iron homeostasis.

For research use only, not for clinical use.