Fd-GOGAT | Ferredoxin-dependent Glutamate synthase

Inquiry

Cat: PA00624

Size: 100 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Spinacia oleracea, Synechocystis sp. PCC6803, Zea mays

Datasheet:

Product Info

Immunogen

Purified full length, tag cleaved, recombinant Zea mays GOGAT, UniProt: P23225

Host

Rabbit

Clonality

Polyclonal

Purity

Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.

Format

Liquid at 2 mg/ml.

Storage

Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

ELISA (ELISA), Western blot (WB)

Recommended dilution

1: 2000 - 1: 5000 (WB)

Expected | apparent MW

175 kDa (Zea mays), 168 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Spinacia oleracea, Synechocystis sp. PCC6803, Zea mays

Predicted reactivity

Arthrospira platensis

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Description

Glutaminoxy-glutarate aminotransferase (GOGAT) is an enzyme involved in the synthesis of glutamate from glutamine and alpha-ketoglutarate. There are two forms of GOGAT in plants: ferridoxin dependent GOGAT (Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT). 95% of GOGAT in plants are Fd-GOGAT type. Fd-GOGAT is encoded in Arabidopsis by two genes, glu1 and glu2. Fd-GOGAT(both forms) is highly conserved in plants, red algae and cyanobacteria. Ferridoxin dependent glutamate synthetase (Fd-GOGAT) is involved in the biosynthesis of glutamate in leaves. This protein is necessary for the re-assimilation of ammonium ions produced during photorespiration. The gene name is GlsF.

For research use only, not for clinical use.