F-box protein VBF (VBF), Recombinant Protein
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F-box protein VBF (VBF), Recombinant Protein

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Cat: RP00150
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana F-box protein VBF (VBF)
Product Gene Name
VBF recombinant protein
Product Synonym Gene Name
VBF
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MMMLPEACIA NILAFTSPAD AFSSSEVSSV FRLAGDSDFV WEKFLPSDYK SLISQSTDHH WNISSKKEIY RCLCDSLLID NARKLFKINK FSGKISYVLS ARDISITHSD HASYWSWSNV SDSRFSESAE LIITDRLEIE GKIQTRVLSA NTRYGAYLIV KVTKGAYGLD LVPAETSIKS KNGQISKSAT YLCCLDEKKQ QMKRLFYGNR EERMAMTVEA VGGDGKRREP KCRDDGWMEI ELGEFETREG EDDEVNMTLT EVKGYQLKGG ILIDGIEVRP KT
Sequence Positions
1-282, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
31,840 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
F-box protein

NCBI/Uniprot Data

NCBI Accession #
NP_176021.1
NCBI GI #
15223490
NCBI GenBank Nucleotide #
NM_104504.3
NCBI GeneID
842078
NCBI Official Full Name
phloem protein 2-B14
NCBI Official Symbol
PP2-B14
NCBI Official Synonym Symbols
AtPP2-B14; F14G9.14; F14G9_14; phloem protein 2-B14; VBF; VIP1-binding F-box protein
NCBI Protein Information
phloem protein 2-B14
NCBI Summary
Encodes an F-box protein that can functionally replace VirF, regulating levels of the VirE2 and VIP1 proteins via a VBF-containing SCF complex. It is thought to be involved in DNA integration and T-DNA degradation.
UniProt Gene Name
VBF
UniProt Synonym Gene Names
PP2B14; AtPP2-B14
UniProt Protein Name
F-box protein VBF
UniProt Synonym Protein Names
Protein PHLOEM PROTEIN 2-LIKE B14; AtPP2-B14; VIP1-binding F-box protein
UniProt Primary Accession #
Q9C7K0
UniProt Related Accession #
Q9C7K0
UniProt Comments
Component of SCF(VBF) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins such as VIP1 and Agrobacterium virE2, after their implication in T-DNA translocation to the host nucleus (can functionally replace Agrobacterium VirF). Required during Agrobacterium-induced tumor formation.

For research use only, not for clinical use.