Erythrina Cristagalli Lectin (ECL, ECA), Fluorescein

It has been reported that erythrolectin (ECL) can be used to isolate human natural killer (NK) cells using negative selection planning techniques (protocols are available upon request or on our website). Human NK cells appear to lack the accessible surface carbohydrate structure needed to bind ECL and, unlike other monocytes, do not adhere to ECL-coated petri dishes. Because the process involves a negative selection screening technique, a high recovery rate of live NK cells can be obtained. Adherent cells can also be incubated in galactose or lactose to recover. Fluorescein-labeled erythritum lectins bind appropriate amounts of fluorescent dyes to provide optimal staining properties for the lectins. This conjugated substance basically does not contain unconjugated fluorescent dyes. The excitation maximum is 495nm and the emission maximum is 515nm.