Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound

Inquiry

Cat: PL00695

Size: 2 ml

Sugar Specificity: Galactose, N-Acetylgalactosamine

Datasheet:

Description

Agarose binding erythritum lectin is prepared with 4% agarose beads. Erythrin is composed of two distinct subunits, 28kda and 26kda. Carbohydrate structures that bind to ECL are commonly found in membrane and serum glycoproteins of mammalian origin.

Product Info

Conjugate

Agarose

Sugar Specificity

Galactose, N-Acetylgalactosamine

Storage

2-8℃; Do not freeze

Application

Glycobiology, Affinity Chromatography

Recommended dilution

Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-2100. Alternatively, 0.2 M lactose can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.

Solution

10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM lactose, 0.08% sodium azide

For research use only, not for clinical use.