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Endoribonuclease Dicer homolog 2 (At3g03300), Recombinant Protein
Cat: RP09914
Size: 0.5 mg (E-Coli)/ 0.05 mg (Baculovirus)/ 0.5 mg (Yeast)/ 0.05 mg (Mammalian-Cell)/ 1 mg (E-Coli)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 0.5 mg (Baculovirus)/ 1 mg (Baculovirus)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Endoribonuclease Dicer homolog 2 (At3g03300) , partial
Product Gene Name
At3g03300 recombinant protein
Product Synonym Gene Name
At3g03300
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
ALEKAIKQNT IVFLETGSGK TLIAIMLLRS YAYLFRKPSP CFCVFLVPQV VLVTQQAEAL KMHTDLKVGM YWGDMGVDFW DSSTWKQEVD KYEVLVMTPA ILLDALRHSF LSLSMIKVLI VDECHHAGGK HPYACIMREF YHKELNSGTS NVPRIFGMTA SLVKTKGENL DSYWKKIHEL
Sequence Positions
31-210, Partial, provide the Helicase ATP-binding domain.
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
155,286 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Endoribonuclease
NCBI/Uniprot Data
NCBI Accession #
NP_001078101.1
NCBI GI #
145331748
NCBI GenBank Nucleotide #
NM_001084632.1
NCBI GeneID
821300
NCBI Official Full Name
dicer-like 2
NCBI Official Symbol
DCL2
NCBI Official Synonym Symbols
ATDCL2; dicer-like 2; DICER-LIKE 2; T17B22.28
NCBI Protein Information
dicer-like 2
NCBI Summary
Encodes a Dicer-like protein that functions in the antiviral silencing response in turnip-crinkle virus-infected plants but not in TMV or CMV-strain-Y-infected plants. Involved in the production of ta-siRNAs. Partially antagonizes the production of miRNAs by DCL1. Substitutes for DCL4 to produce viral siRNA when DCL4 is missing or inhibited. Able to produce siRNAs but not miRNAs.
UniProt Gene Name
At3g03300
UniProt Synonym Gene Names
AtDCL2
UniProt Protein Name
Endoribonuclease Dicer homolog 2
UniProt Synonym Protein Names
Dicer-like protein 2; AtDCL2
UniProt Primary Accession #
Q3EBC8
UniProt Secondary Accession #
Q1KL57; Q1KL58; Q9M9P8
UniProt Related Accession #
Q3EBC8
UniProt Comments
Ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Involved in the processing of natural small interfering RNAs (nat-siRNAs, derived from cis-natural antisense transcripts) by cleaving small dsRNAs into 24 nucleotide nat-siRNAs. Plays an essential role in transitive silencing of transgenes by processing secondary siRNAs. This pathway, which requires DCL4 and RDR6, amplifies silencing by using the target RNA as substrate to generate secondary siRNAs, providing an efficient mechanism for long-distance silencing. May participate with DCL3 in the production of 24 nucleotide repeat-associated siRNAs (ra-siRNAs) which derive from heterochromatin and DNA repeats such as transposons. Plays a role in antiviral RNA silencing. Involved in the production of viral siRNAs derived from the turnip crinkle virus (TCV) and tobacco rattle virus (TRV). Targeted by the viral silencing suppressor (VSR) protein 2b of the cucumber mosaic virus (CMV) that inactivates DCL2 function in RNA silencing. Does not seem to be involved in microRNAs (miRNAs) processing.
For research use only, not for clinical use.