Endoribonuclease Dicer homolog 1 (DCL1), Recombinant Protein
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Endoribonuclease Dicer homolog 1 (DCL1), Recombinant Protein

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Cat: RP01146
Size: 0.05 mg (E-Coli)/ 0.05 mg (Yeast)/ 0.2 mg (E-Coli)/ 0.5 mg (E-Coli)/ 0.05 mg (Baculovirus)/ 0.2 mg (Yeast)/ 0.1 mg (Baculovirus)/ 0.5 mg (Yeast)/ 0.05 mg (Mammalian-Cell)/ 1 mg (E-Coli)/ 0.5 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (B
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Endoribonuclease Dicer homolog 1 (DCL1) , partial
Product Gene Name
DCL1 recombinant protein
Product Synonym Gene Name
DCL1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
PMHPVRELQE RCQQQAEGLE YKASRSGNTA TVEVFIDGVQ VGVAQNPQKK MAQKLAARNA LAALKEKEIA ESKEKHINNG NAGEDQGENE NGNKKNGHQP FTRQTLNDIC LRKNWPMPSY RCVKEGGPAH AKRFTFGVRV NTSDRGWTDE CIGEPMPSVK KAKDSAAVLL LELLNKTFS
Sequence Positions
1731-1909. Fragment at the C-terminal, including the DRBM 1 & DRBM 2 Domain
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
213,574 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Endoribonuclease

NCBI/Uniprot Data

NCBI Accession #
NP_171612.1
NCBI GI #
15223286
NCBI GenBank Nucleotide #
NM_099986.4
NCBI GeneID
839574
NCBI Official Full Name
dicer-like 1
NCBI Official Symbol
DCL1
NCBI Official Synonym Symbols
ABNORMAL SUSPENSOR 1; ASU1; ATDCL1; CAF; CARPEL FACTORY; dicer-like 1; DICER-LIKE 1; EMB60; EMB76; EMBRYO DEFECTIVE 60; EMBRYO DEFECTIVE 76; SHORT INTEGUMENTS 1; SIN1; SUS1; SUSPENSOR 1; T25K16.4; T25K16_4
NCBI Protein Information
dicer-like 1
NCBI Summary
Encodes a Dicer homolog. Dicer is a RNA helicase involved in microRNA processing. Mutations in this locus can result in embryo lethality. Embryo shape at seed maturity is globular-elongate. Other mutants convert the floral meristems to an indeterminate state, others yet show defects in ovule development. mRNA is expressed in all shoot tissues. DCL1 is able to produce miRNAs and siRNAs.
UniProt Gene Name
DCL1
UniProt Synonym Gene Names
ASU1; CAF SIN1; SUS1; AtDCL1
UniProt Protein Name
Endoribonuclease Dicer homolog 1
UniProt Synonym Protein Names
Dicer-like protein 1; AtDCL1; Protein ABNORMAL SUSPENSOR 1; Protein CARPEL FACTORY; Protein SHORT INTEGUMENTS 1; Protein SUSPENSOR 1
UniProt Primary Accession #
Q9SP32
UniProt Secondary Accession #
Q9FDY6; Q9MAN0
UniProt Related Accession #
Q9SP32
UniProt Comments
Ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Functions with DRB1/HYL1 and SERRATE proteins for accurate pri-miRNAs to miRNAs processing. Indirectly involved in the production of trans-acting small interfering RNAs (ta-siRNAs) derived from the TAS1, TAS2 or TAS3 endogenous transcripts by participating in the production of their initiating miRNAs. Involved in the processing of natural siRNAs (nat-siRNAs, derived from cis-natural antisense transcripts) by cleaving 24 nucleotide nat-siRNAs into 21 nucleotide nat-siRNAs. Can produce RDR6-dependent endogenous ta-siRNAs derived from TAS1 and TAS2. Required for the production of 30-40 nucleotide bacterial-induced long siRNAs (lsiRNA). Acts redundantly with DICER-LIKE 3 (DCL3) to promote flowering via repression of FLOWERING LOCUS C (FLC). Represses antiviral RNA silencing through negative regulation of the expression of DCL4 and DCL3.

For research use only, not for clinical use.