EIN3 | Ethylene insensitive 3

Inquiry

Cat: PA00250

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

Recombinant EIN3 of Arabidopsis thaliana , UniProt: O24606, TAIR: At3g20770

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Storage

Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

71,4 | 78 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Populus trichocarpa

Not reactive in

Medicago truncatula

Additional information

Western blot condtions for anti-EIN3 antibodies are described in Smalle et al. 2002.Recommended membrane for blocking: nitrocellulose, blocking reagent: 10 % non-fat milk, antibody incubation buffer: PBS-T.

Description

EIN3(ethylene insensitivity 3) plays a positive regulatory role in the ethylene reaction pathway and is necessary for the response of adult plant tissues to ethylene. Activated by phosphorylation of MPK3 and MPK6 (PubMed:18273012). It is down-regulated by KIN10 to control its protein stability in a phosphorylation-dependent manner. Also known as: protein ethylene insensitive

For research use only, not for clinical use.