eEF1a | Elongation factor 1-alpha
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eEF1a | Elongation factor 1-alpha

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Cat: PA00237
Size: 50 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Avena sativa, Fraxinus pennsylvanica, Hordeum vulgare, Olea europaea, Oryza sativa, Physcomitrium patens, Solanum tuberosum, Zea mays
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide derived from known sequences of eEF1a including Arabidopsis thaliana EF-1-alpha 1P0DH99 AT1G07940  EF-1-alpha 2 Q8W4H7  AT1G07930, EF-1-alpha 3 Q0WL5 AT1G07920, EF-1-alpha 4 Q8GTY0 AT5G6039
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
49,5 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Avena sativa, Fraxinus pennsylvanica, Hordeum vulgare, Olea europaea, Oryza sativa, Physcomitrium patens, Solanum tuberosum, Zea mays
Predicted reactivity
Daucus carota, Euglena gracilis (and unicellular flagellate protists) , Glycine max, Lilium longiflorum, Malus domestica, Nicotiana tabacum, Pisum sativum, Solanum lycopersicum, Triticum aestivum,  Vicia faba
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

This product can be sold containing ProClin if requested.Following protein resolubilization with urea/thiourea/CHAPS signal strength of recognized band is decreased. Recommended protein extraction conditions are without urea.

Description

EF1A(Extension factor 1-α) belongs to the gtp binding extension factor family and is localized in the cytoplasm. It is an essential enzyme in the extension phase of protein synthesis. There are four genes encoding EF1A in Arabidopsis Thaliana, with genes 1, 2 and 3 having the same sequence. Synonym :eEF-1A.

For research use only, not for clinical use.