Immunogen
KLH-conjugated synthetic peptide: DYKDDDDK (Sigma FLAG )
Purity
Immunogen affinity purified serum, in PBS pH 7.4.
Reconstitution
For reconstitution add 50 µl, of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 1000 - 1: 5000 (WB)
Expected | apparent MW
Depends upon fusion protein partner