DYKDDDDK, HRP conjugated (Clone M2)

Inquiry

Cat: PA00013

Size: 100 µg

Host: Mouse

Clonality: Monoclonal

Datasheet:

Product Info

Immunogen

DYKDDDDK (Sigma FLAG )

Host

Mouse

Clonality

Monoclonal

Purity

Protein A purified.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Storage

Store freeze-dried poweder at 2-8°C.Rehydrate with 1.0 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily. Shelf life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.

Application

Western blot (WB)

Recommended dilution

100 ng/ml (ELISA), 1 : 20-1 : 50 (ICC), 1 : 20-1 : 50 (IF), 3 µg (IP), 1 : 10-1 : 50 (WB)

Reactivity

Additional information

Antibody is provided in: 100 mM NaPO4 (pH 7,4), 100 mM NaCl, 1,5% BSA, 50 mM Sucrose, 0,01 % Thimerosal,Affinity purified antibody has over 95% purity based on SDS-PAGE

Description

Mouse anti-DykDDDDK is a primary antibody that binds DYKDDDDK (Sigma FLAG) and is directly coupled to HRP(horseradish peroxidase). This is due to the advantage of shortening the assay time by not requiring the use of DYKDDDDK's secondary antibody.

For research use only, not for clinical use.