DYKDDDDK (binds to Sigma FLAG , clone FG4R)

Inquiry

Cat: PA00016

Size: 50 µg

Host: Mouse

Clonality: Monoclonal

Confirmed reactivity: N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins

Datasheet:

Product Info

Immunogen

KLH-conjugated DYKDDDDK (FLAG) synthetic peptide

Host

Mouse

Clonality

Monoclonal

Purity

Total IgG. Protein A purified in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.

Format

Liquid

Storage

Store at -20°C for up to 2 years.

Application

Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution

1 : 1000-5000 (1-5ng) (WB) (incubate for one hour at room temperature), 1 : 500-1 :2000 (IS) For best results with other assays (Dot, ELISA, IP), please determine optimal working dilution by end user

Reactivity

Confirmed reactivity

N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Working dilution for Dot Blot, ELISA and IP needs to be determined experimentally.Antibody is purified on Protein A.

Description

FLAG-tag is a marker that can be added to the protein sequence motif DYKXXD. It can be used for affinity chromatography to isolate recombinant proteins from wt proteins. It can also be used to separate protein complexes with multiple subunits.

For research use only, not for clinical use.