DNA topoisomerase 1 (TOP1), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana DNA topoisomerase 1 (TOP1) , partial

Product Gene Name

TOP1 recombinant protein

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

102,799 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

DNA topoisomerase

NCBI Accession #

NP_200341.1

NCBI GI #

15240492

NCBI GenBank Nucleotide #

NM_124912.5

NCBI GeneID

835623

NCBI Official Full Name

DNA topoisomerase I alpha

NCBI Official Symbol

TOP1ALPHA

NCBI Official Synonym Symbols

DNA topoisomerase 1 beta; DNA topoisomerase I alpha; MGO1; MGOUN 1; MTE17.1; MTE17_1; TOP1; TOP1BETA; TOPOISOMERASE 1

NCBI Protein Information

DNA topoisomerase I alpha

NCBI Summary

Encodes a type-I DNA topoisomerase I. Disruptions in this gene affect phyllotaxis and plant architecture suggesting that the gene plays a critical role in the maintenance of a regular pattern of organ initiation. Isolated as a protein oxidized during seed germination; proteomics approach revealed differences in de novo synthesis levels of this protein in condition with vs. without salicylic acid in the period from 0 to 40 hrs. following seed imbibition. Functions in stem cell maintenance at all stages of shoot and floral meristems and in the regulation of gene silencing.

UniProt Gene Name

TOP1A

UniProt Protein Name

DNA topoisomerase 1 alpha

UniProt Synonym Protein Names

DNA topoisomerase 1

UniProt Primary Accession #

P30181

UniProt Secondary Accession #

Q56XA9; Q8RX06

UniProt Related Accession #

P30181

UniProt Comments

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone . Can complement a TOP1-deficient yeast mutant (PubMed:16669064). Plays a critical role in the maintenance of a regular pattern of organ initiation. Topoisomerases I enzymes (TOP1A and TOP1B) are essential for plant survival (PubMed:12215507). Functions together with the stem cell maintenance gene WUSCHEL (WUS) in stem cell regulation. Required to maintain developmentally regulated gene repression. Functions synergistically with chromatin remodeling factors (PubMed:20228247). Is required for the repression of WUS expression in flower development. Plays a role in polycomb group (PcG) protein-mediated histone H3 trimethylation on 'Lys-27' (H3K27me3) at the WUS gene locus. H3K27me3 induces transcriptional repression of WUS. May assist AGAMOUS (AG) in recruiting PcG proteins to WUS locus. Reduces nucleosome density, especially at genes that are targets of PcG proteins (PubMed:25070639). Plays a role in epigenetic silencing. Involved in RNA-directed DNA methylation (RdDM) by promoting Pol V transcription to generate long non-coding RNA transcripts. Is dispensable for Pol IV-mediated small interfering RNA (siRNA) biogenesis. Promotes transposable element (TE) silencing at endogenous RdDM target loci through histone H3 dimethylation of 'Lys-9' (H3K9me2). Promotes the production of Pol V-dependent long non-coding transcripts that facilitate the recruitment of siRNA-AGO4 and AGO4 occupancy at TEs (PubMed:24992598).