DNA topoisomerase 1 (TOP1), Recombinant Protein

Full Product Name

Recombinant Daucus carota DNA topoisomerase 1 (TOP1), partial

Product Gene Name

TOP1 recombinant protein

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

89,771 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

DNA topoisomerase

NCBI Accession #

P93119.1

NCBI GI #

14285799

NCBI Official Full Name

DNA topoisomerase 1

UniProt Gene Name

TOP1

UniProt Protein Name

DNA topoisomerase 1

UniProt Synonym Protein Names

DNA topoisomerase I

UniProt Primary Accession #

P93119

UniProt Comments

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone ().