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DNA repair helicase XPB2 (XPB2), Recombinant Protein
Cat: RP01367
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana DNA repair helicase XPB2 (XPB2) , partial
Product Gene Name
XPB2 recombinant protein
Product Synonym Gene Name
XPB2
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
86,736 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
DNA repair helicase
NCBI/Uniprot Data
NCBI Accession #
NP_568591.1
NCBI GI #
18422089
NCBI GenBank Nucleotide #
NM_123501.3
NCBI GeneID
834138
NCBI Official Full Name
homolog of Xeroderma pigmentosum complementation group B 2
NCBI Official Symbol
XPB2
NCBI Official Synonym Symbols
ARABIDOPSIS HOMOLOG OF XERODERMA PIGMENTOSUM COMPLEMENTATION GROUP B 2; ATXPB2; homolog of Xeroderma pigmentosum complementation group B 2; MYC6.7; MYC6_7
NCBI Protein Information
homolog of Xeroderma pigmentosum complementation group B 2
NCBI Summary
Encodes XPB2, a DNA repair protein and transcription factor. Arabidopsis thaliana has duplicated XPB gene (AtXPB1 and AtXPB2, with high similarity to each other). XPB proteins are involved in both DNA repair and transcription, they are component of the transcription factor IIH (TFIIH) and are responsible for DNA helicase activity during nucleotide (nt) excision repair (NER). Complementation assays in yeast rad25 mutant strains suggest the involvement of AtXPB2 in DNA repair. Although both genes are expressed in a constitutive manner during the plant life cycle, Northern blot analyses suggest that light modulates the expression level of both XPB copies.
UniProt Gene Name
XPB2
UniProt Synonym Gene Names
TFIIH subunit XPB; AtXPB2
UniProt Protein Name
General transcription and DNA repair factor IIH helicase subunit XPB2
UniProt Synonym Protein Names
ERCC3 homolog 2; RAD25 homolog 2; AtXPB2; XPB homolog 2
UniProt Primary Accession #
Q9FUG4
UniProt Secondary Accession #
Q9FN64
UniProt Related Accession #
Q9FUG4
UniProt Comments
ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
For research use only, not for clinical use.