DISCONTINUED Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (higher plants)
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DISCONTINUED Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (higher plants)

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Cat: PA00227
Size: 50 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Echinochloa crus-galli, Hordeum vulgare, Nicotiana tabacum, Panicum miliaceum, Picea abies, Pisum sativum, Thermosynechococcus elongatus, Solanum lycopersicum, Synechocystis sp. PCC6803, Zea mays
Datasheet:

Product Info

Immunogen
maize cytochrome f purified from chloroplasts, including a final gel purification on a denaturing gel. protein used to elicit this antibody is conserved in Arabidopsis thaliana cyt f UniProt: P56771, TAIR: AtCg00540
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Application
Immunogold (IG), Western blot (WB)
Recommended dilution
1 : 120 (IG), 1 : 2500-1 : 5000 (WB)
Expected | apparent MW
31-32 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Echinochloa crus-galli, Hordeum vulgare, Nicotiana tabacum, Panicum miliaceum, Picea abies, Pisum sativum, Thermosynechococcus elongatus, Solanum lycopersicum, Synechocystis sp. PCC6803, Zea mays
Predicted reactivity
Brachypodium distachyon, Cannabis sativa, Cucumis melo, Oryza sativa, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.

Additional information

This antibody can be used as a marker for the soluble and insoluble fractions Rühle et al. (2021). Can be provided with 0.1% ProClin, please inquire.,This antibody can be used to identify di and monomeric cytb6-f complexes  Kaňa et al. 2020.

Description

cytb6/f multisubunit complex is an important component of photosynthetic electron transport chain in higher plants, green algae and cyanobacteria. This complex catalyzes the oxidation of quinoline and the reduction of plastocyanin. This reaction allows the establishment of the proton force required for ATP synthesis. The complex consists of four main subunits: the petA gene product corresponding to the C-type cytochrome (cytf), the petB gene product corresponding to the b /c 'type cytochrome (cyt b6) with three hemoglobin proteins, and the PETA gene product corresponding to the three hemoglobin type. petD gene products (subunit IV or suIV) and petC gene products corresponding to Rieske/ iron/thiopprotein.

For research use only, not for clinical use.