Dendra2 tag

Dendra2, a modified version of the green-to-red photoconverting protein Dendra that evolved from the eight corals (Dendronephthya), shows brighter fluorescence before and after the photoconversion. The excitation maximum of Dendra2 is 490 nm before and 553 nm after light activation, and its emission maximum is 507 nm before and 573 nm after light activation. The light that activates Dendra2 is UV/ purple to blue. The non-activated Dendra2 has similar spectral characteristics to EGFP, and this green fluorescence can also be detected at low light intensity blue light. At the same high intensity of blue (or ultraviolet/UV) light, Dendra2 is activated by light and acquires emission characteristics similar to TRITC.