Delta-VPE | Vacuolar-processing enzyme delta-isozyme
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Delta-VPE | Vacuolar-processing enzyme delta-isozyme

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Cat: PA01669
Size: 100 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana
Datasheet:

Product Info

Immunogen
Recombinant, His6-tagged, delta-VPE from Arabidopsis thaliana, UniProt: Q9LJX8, TAIR: At3g20210
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Format
Liquid at 2 mg/ml.
Storage
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunofluorescence (IF), Immunolocalisation (IL), Western blot (WB)
Recommended dilution
1: 500 (IF), 1: 5000 (IL), 1: 5000 (WB)
Expected | apparent MW
52 kDa (inactive precursor) | 37-38 kDa (mature, active form)

Reactivity

Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativus
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Subcellular localisation is seed specific and restricted to developing seeds at 7 days after anthesis, Delta-VPE is detected at lower levels in flowers siliques, specifically in seed coats

Description

Delta-VPE (cavitation processing enzyme Delta-isoenzyme) is a asparagine-specific endopeptidase that may be involved in processing proteins that target cavitation (through similarity). This enzyme may be involved in post-translational proteolysis of seed storage proteins in developing seed storage protein vacuoles (PubMed:12417707, PubMed:14688293). It showed caspase-1-like activity in extracellular particles. During the early stages of seed development, the seed coat is formed by modulating cell death within specific cell layers (Nakaune et al. 2005).

For research use only, not for clinical use.