Deg1 | chloroplastic DegP-type serine protease 1

Inquiry

Cat: PA00850

Size: 360 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Pisum sativum, Zea mays

Datasheet:

Product Info

Immunogen

Recombinant Deg1 of Arabidopsis thaliana O22609, At3g27925, overexpressed in E.coli

Host

Rabbit

Clonality

Polyclonal

Purity

Total IgG. Protein G purified in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 200 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 500/5 ug thylakoid fraction (WB)

Expected | apparent MW

46,6 | 36 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Pisum sativum, Zea mays

Predicted reactivity

Ricinus communis

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Unspecific reaction at ca, 30 kDa and ca, 85 kDa (checked by by mass spec, and confirmed not to be neither Deg1 nor any other protease, The band at the right MW does contain Deg1 by our MS analysis

Description

Deg1 belongs to the Arabidopsis S1 curd trypsin family, which contains 16 protein members. Deg1 is localized in chloroplasts.

For research use only, not for clinical use.