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DEAD-box ATP-dependent RNA helicase 2 (RH2), Recombinant Protein
Cat: RP00252
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana DEAD-box ATP-dependent RNA helicase 2 (RH2)
Product Gene Name
RH2 recombinant protein
Product Synonym Gene Name
RH2
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MATANPGRGG GRRGGGAMDD DKLVFETTDG IEPITSFNDM GIKEDVLRGV YEYGFEKPSA IQQRAVMPIL QGRDVIAQAQ SGTGKTSMIA LSVCQVVDTS SREVQALILS PTRELATQTE KTIQAIGLHA NIQAHACIGG NSVGEDIRKL EHGVHVVSGT PGRVCDMIKR RSLRTRAIKL LILDESDEML SRGFKDQIYD VYRYLPPDLQ VCLVSATLPH EILEMTSKFM TEPVKILVKR DELTLEGIKQ FFVAVEKEEW KFDTLCDLYD TLTITQAVIF CNTKRKVDYL SEKMRSHNFT VSSMHGDMPQ KERDAIMNEF RSGDSRVLIT TDVWARGIDV QQVSLVINYD LPNNRELYIH RIGRSGRFGR KGVAINFVKS DDIKILRDIE QYYSTQIDEM PMNVADLI
Sequence Positions
1-408, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
45,845 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Opsin
NCBI/Uniprot Data
NCBI Accession #
NP_188610.1
NCBI GI #
15230945
NCBI GenBank Nucleotide #
NM_112866.4
NCBI GeneID
821513
NCBI Official Full Name
eukaryotic initiation factor 4A-III
NCBI Official Symbol
EIF4A-III
NCBI Official Synonym Symbols
eukaryotic initiation factor 4A-III
NCBI Protein Information
eukaryotic initiation factor 4A-III
NCBI Summary
Encodes an RNA helicase that may be a component of the Exon Junction Complex. Subcellular localization is modulated by stress. Under normal conditions it is localized to the nuceloplasm but under hyopoxic conditions it localizes to the nucleolus and splicing speckles.
UniProt Gene Name
EIF4A3
UniProt Synonym Gene Names
AtRH02
UniProt Protein Name
Eukaryotic initiation factor 4A-III homolog
UniProt Synonym Protein Names
DEAD-box ATP-dependent RNA helicase 2
UniProt Primary Accession #
Q94A52
UniProt Secondary Accession #
Q9ZS14
UniProt Related Accession #
Q94A52
UniProt Comments
ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by MLN51/CASC3, but abolished in presence of the MAGO-Y14 heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGO-Y14 heterodimer increases the RNA-binding affinity of the EJC . Plays a role in abiotic stress adaptation. Can regulate abiotic stress resistance partially via the control of acetoacetyl-CoA thiolase 2 (AC Q8S4Y1) expression (PubMed:26883227).
For research use only, not for clinical use.