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DCL3 | Dicer-like protein 3
Cat: PA01338
Size: 200 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana
Datasheet:
Product Info
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DCL3 sequence, Uniprot: Q9LXW7 , TAIR: At3g43920
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 200 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
117 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Arabidopsis thaliana
Not reactive in
Zea mays
Additional information
Recommend protein load is from 40-50 µg/well (using 1,5 mm spacers helps to obtain wider wells)
Description
DCL3 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). By cutting primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs) play a role in the microRNAs (miRNAs) biogenetic pathway. Dicer homolog of endonuclease
For research use only, not for clinical use.