Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (algal)

Cat: PA00538

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorella vulgaris, Colobanthus quitensis Kunt Bartl, CRaterostigma pumilum, Hordeum vulgare, Nannochloropsis gaditana, Nicotiana tabacum, Nostoc sp. PCC7120, Physcomitrium patens, Selaginella martensi, Synechocystis sp. 6803 substrain PCC-M, Ulva prolifera

Datasheet:

Product Info

Immunogen

GST fusion to cytochrome f from Chlamydomonas reinhartii P23577

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 2000-1 : 50 000 (WB)

Expected | apparent MW

34 | 31-32 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorella vulgaris, Colobanthus quitensis Kunt Bartl, CRaterostigma pumilum, Hordeum vulgare, Nannochloropsis gaditana, Nicotiana tabacum, Nostoc sp. PCC7120, Physcomitrium patens, Selaginella martensi, Synechocystis sp. 6803 substrain PCC-M, Ulva prolifera

Predicted reactivity

Algae, Citrus x limon, Zea mays

Additional information

Contains 0,02 % sodium azide as preservative,Reaction with cyanobacteria: Synechocystis 6803 and Synechococcus 7942 possible to obtain on total cell extract when using antibody at 1: 500 and longer exposure time.

Description

cytb6/f multisubunit complex is an important component of photosynthetic electron transport chain in higher plants, green algae and cyanobacteria. This complex catalyzes the oxidation of quinoline and the reduction of plastocyanin. This reaction allows the establishment of the proton force required for ATP synthesis. The complex consists of four main subunits: the petA gene product corresponding to the C-type cytochrome (cytf), the petB gene product corresponding to the b /c 'type cytochrome (cyt b6) with three hemoglobin proteins, and the PETA gene product corresponding to the three hemoglobin type. petD gene products (subunit IV or suIV) and petC gene products corresponding to Rieske/ iron/thiopprotein.

For research use only, not for clinical use.