Cyt c | Cytochrome c
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Cyt c | Cytochrome c

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Cat: PA00437
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Brassica oleracea, Glycine max, Pisum sativum, Zea mays
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana cytochrome c protein sequence, UniProt:D7KMK0 (C-1) D7LY03 (C-2), TAIR: At1g22840 (Cytc1) and At4g10040 (Cytc2)
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles and Store at -80°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunolocalization (IL), Western blot (WB)
Recommended dilution
1: 100 (IL), 1 : 5000 (WB)
Expected | apparent MW
12.5 | 14 kDa (for Arabidopsis thaliana)

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Brassica oleracea, Glycine max, Pisum sativum, Zea mays
Predicted reactivity
cytc1 and cytc2 from following species: A. theoprasi, Brassica napus, Brassica oleracea, Cannabis sativa, C. maxima, Chlamydomonas reinhardtii (peptide target partially conserved), Lupinus luteus,  Medicago truncatula, Nicotiana tabacum, Oryza sativa, Ostreococcus (peptide target partially conserved), P. aurea, Physcomitrium patens, Ricinus communis, S. nigra, Solanum lycopersivum, Vitis vinifera.
Not reactive in
Arabidopsis thaliana CytC6, Chinesecuscuta sp.

Additional information

The presence of cytochrome c in the cysotol is a marker of PCD (programmed cell death)

Description

Cytochrome c is located in the inner mitochondrial membrane. It is a small heme protein, and unlike other cytochrome, it is highly soluble. This protein is an important part of the electron transport chain, which oxidizes and reduces in the absence of bound oxygen.

For research use only, not for clinical use.