COXII | Plant Cytochrome oxidase subunit II (affinity purified)

Cat: PA00432

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Actinidia chinensis, Arabidopsis thaliana (leaf extract and isolated mitochondria), Betula nana, Brassica napus, Brassica oleracea, Cicer aretinum, Cucumis melo, Cucumis sativus, Erophorum vaginatum, Hordeum vulgare, Lilium longifolorum, Nicotiana tabacum, Picea abies, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica, Physcomitrium patens, Triticum aestivum, Triticum durum Desf. , Zea mays, Vicia faba, Quercus rubra

Datasheet:

Product Info

Immunogen

KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Blue Native-PAGE (BN-PAGE), Western blot (WB)

Recommended dilution

1 : 1000 (BN-PAGE), 1 : 1000 (WB)

Expected | apparent MW

29.4 | 30 kDa (for Arabidopsis thaliana)

Reactivity

Confirmed reactivity

Actinidia chinensis, Arabidopsis thaliana (leaf extract and isolated mitochondria), Betula nana, Brassica napus, Brassica oleracea, Cicer aretinum, Cucumis melo, Cucumis sativus, Erophorum vaginatum, Hordeum vulgare, Lilium longifolorum, Nicotiana tabacum, Picea abies, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica, Physcomitrium patens, Triticum aestivum, Triticum durum Desf. , Zea mays, Vicia faba, Quercus rubra

Predicted reactivity

Cucumis melo, Glycine max, Oryza sativa, Physcomitrium patens, Pisum sativum, Triticum aestivum, Vigna radiata

Not reactive in

Saccharina japonica

Additional information

Cellular [compartment marker] of mitochondrial inner membrane,Antibody detects COXII protein most optimally in membrane fractions. The signal is weak in a in total protein extract. Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness is 2 mm with 4.5-16 % gradient.

Description

Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain within the mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers electrons from cytochrome c to the bimetallic center of catalytic subunit 1 through the binuclear copper A center. Alias: Cytochrome c oxidase subunit 2

For research use only, not for clinical use.